| Structural Analysis of
Starvation Transmission Electron Microscopy is being
used to examine cells for structural alterations that are associated
with a transition to starvation. The TEM images can potentially complement
proteomics by providing visual clues into changes in protein-based
structures that might be reflected in the proteome, and broader changes
that reflect cell state, but may not be captured in the proteome analysis. |
| Non-stressed, growing cells |
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image
Possible carboxysome-like supramolecular aggregatesclustering near the
inner face of the cytoplasmic membrane
|
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image
Multilamellar membranes, characteristic of N. europaea surround the
cells
|
| Cells starved for ammonium for 2
weeks |
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Loosening of multilamellar membranes, electron dense bodies
form |
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Invagination of multilamellar membranes, multiple electron dense
bodies |
| Cells starved for ammonium
for 2 months |
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Multilamellar membranes degenerate |
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Zoom image of membrane degeneration TEM images produced in association with Randall Massey
(UW-Madison Medical College, Electron Microscopy Facility) |
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